Human Neutrophil Lipocalin

Human Neutrophil Lipocalin (HNL) also named Lipocalin-2 (LCN2) or Neutrophil Gelatinase Associated Lipocalin (NGAL) is a protein that is stored in and secreted from the secondary granules of human neutrophil granulocytes. As indicated by the name HNL belongs to the protein superfamily of lipocalins. The biological function of HNL is uncertain although the protein has been shown to bind a siderophore and therefore potentially acts as an iron-transporting protein. Among inflammatory cells HNL is uniquely found in neutrophils. The production of HNL may be induced by certain epithelial cells e.g. tubular cells in the kidney. HNL exists in biological fluids in several different forms such as monomer, dimers, heteromers, which partly have different cellular origins.

We provide you with the following tools to study the biology of HNL

  • The mouse monoclonal antibody against HNL mab 697 of the IgG1-type is suitable for the identification of HNL in tissues by immunocytochemistry (available). We can also provide you with several other monoclonal antibodies against EPX/EDN with different epitope specificities and which may be used for the development of immunoassays of HNL ro for Western blotting. For immunohistochemistry the best results may be obtained by mixing several different antibodies together.
  • We also have available a specific rabbit polyclonal antibody
  • Native HNL was purified from the granules of neutrophils of healthy subjects (available)
  • An ELISA assay for the measurement HNL in various body fluids (available)