Eosinophil Cationic Protein

Eosinophil Cationic Protein

ECP is a very basic protein that is stored in and secreted from human eosinophil granulocytes. ECP is a multifunctional protein of which the cytotoxic activity may be the most conspicous. ECP may be measured in blood and other body fluids as a reflection of the turnover and activity of the eosinophil population. Small amounts of ECP may be found in neutrophil granulocytes.

ECP exists in two forms, the cytotoxic and the non-cytotoxic form. These two different forms are the consequence of a single nucleotide polymorphism (SNP) in the coding region of the ECP-gene sequence at position 434. The SNP coding for the cytotoxic ECP form is the most common in the Scandinavian population whereas the SNP coding for the non-cytotoxic form dominates in populations living in tropical areas of Africa. In addition to these forms determined by the SNP, ECP also exists in many different glycosylated forms with differences in biological activities.

The mouse monoclonal antibody EG2 of the IgG1-type is suitable for the identification of ECP in tissues by immunocytochemistry (available). EG2 may also recognize an epitope in EPX/EDN. We have several other monoclonal antibodies, which are epitope specific to ECP (e.g. Clone 614) and also neutralize the cytotoxic activity of ECP (Clone 562).

We can also provide you with a specific rabbit polyclonal antibody

All antibodies are suitable for immunocytochemistry, western blotting and immunoassay development

The ECP protein is provided in several forms:
1. Native ECP purified from the granules of healthy scandinavian subjects. The preparation is a mix of all different ECP forms, both cytotoxic and non-cytotoxic (available)
2. Recombinant, cytotoxic ECP with arginine at position 97 produced by insect cells (available)
3. Recombinant, non-cytotoxic ECP with threonine at position 97 produced by insect cells (available)

The ELISA assay for the measurement of ECP in various body fluids is produced in two formats:
1. An ECP assay that measures all forms of ECP
2. An ECP assay that preferentially measures the cytotoxic forms of ECP